pel

pectate lyase C

Locus: BSU_07560

Isoelectric point: 8.42

Molecular weight: 45.34 kDa

Protein length: 420 aa

Gene length: 1263 bp

Function: degradation of polygalacturonic acid

Product: pectate lyase C

Essential: no

E.C.: 4.2.2.2

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
• Groups of genes → Secreted proteins

Gene

Coordinates: 827,993 829,255

The protein

Catalyzed reaction/ biological activity

• Eliminative cleavage of (14)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to UniProt)

Protein family

• lyase 1 family (according to UniProt)

Structure

• 2O1D (bound to trisaccharide), 1BN8, 5X2I

Localization

• extracellular (signal peptide), major constituent of the secretome PubMed

Expression and Regulation

Operons

• Genes: pel
  Description: PubMed

  Regulatory mechanism

  • CcpA: repression, in CcpA regulon
  • TnrA: repression, PubMed, in TnrA regulon
  • ComA: activation, (ComA) PubMed, in ComA regulon

  Regulation

  • expressed at high cell density (ComA) PubMed

Biological materials

Mutant

• MGNA-C248 (pel::erm), available at the NBRP B. subtilis, Japan
• BKE07560 (pel::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCACTATGTACCCCCAT, downstream forward: _UP4_TAAGAAAGTGAAAAACACAA
• BKK07560 (pel::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCACTATGTACCCCCAT, downstream forward: _UP4_TAAGAAAGTGAAAAACACAA

References

Reviews

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Original publications

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