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pdaC [2019-11-11 14:22:59]
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pdaC [2019-11-11 14:22:59]

peptidoglycan deacetylase C, confers lysozyme resistance to modified cell wall peptidoglycans
Locus
BSU_12100
Isoelectric point
9.10
Molecular weight
53.67 kDa
Protein length
467 aa Sequence Blast
Gene length
1404 bp Sequence Blast
Function
cell wall modification
Product
polysaccharide deacetylase C
Essential
no
Synonyms
yjeA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,281,128 1,282,531

    Phenotypes of a mutant

  • sensitive to lysozyme treatment PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • deacetylates N-acetylmuramic acid (MurNAc) but not GlcNAc from peptidoglycan PubMed

    • Protein family

  • polysaccharide deacetylase family (according to UniProt)

    • Paralogous protein(s)

  • YheN:

    • Domains

  • aa 55 - 140: DUF4163
  • aa 155 - 235: DUF3298
  • NodB homology domain (aa 278-452) (according to UniProt)

    • Structure

  • 2C1G (the NodB domain, from Streptococcus pneumoniae, 47% identity) PubMed
  • 3S5T (the DUF3298 domain, from Bacteroides fragilis, 27% identity)

    • Localization

  • cell membrane (single pass) PubMed

    • Expression and Regulation

    Operons

    Genes
    pdaC
    Description
    PubMed

    Regulatory mechanism

  • WalR: repression, in WalR regulon
    • view in new tab

    Additional information

  • the mRNA is cleaved by RNase III PubMed

    • Biological materials

    Mutant

  • MGNA-A355 (yjeA::erm), available at the NBRP B. subtilis, Japan
  • BKE12100 (pdaC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC, downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
  • BKK12100 (pdaC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC, downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC

    • References

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