fliJ

flagellar protein required for formation of basal body, part of the ATPase complex, regulator of the FliI ATPase

Locus: BSU_16250

Isoelectric point: 8.41

Molecular weight: 17.77 kDa

Protein length: 147 aa

Gene length: 444 bp

Function: movement and chemotaxis

Product: flagellar protein required for formation of basal body

Essential: no

Synonyms: cheF

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Flagellar proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,697,196 1,697,639

The protein

Protein family

• FliJ family (single member, according to UniProt)

Structure

• 3AJW (from S. typhimurium, 19% identity, 63% similarity) PubMed

Localization

• cytoplasm, as part of the flagellum attached to the membrane PubMed

Expression and Regulation

Operons

• Genes: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • SwrA: activation, in SwrA regulon
  • CodY: repression, in CodY regulon
  • DegU: repression, in DegU regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Biological materials

Mutant

• BKE16250 (fliJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTTATTCCTCATTTCC, downstream forward: _UP4_CAAGGCCATTAGGAGCGAAT
• BKK16250 (fliJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTTATTCCTCATTTCC, downstream forward: _UP4_CAAGGCCATTAGGAGCGAAT

References

Reviews

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Original publications

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