yaaJ [2017-09-27 16:04:45]

tRNA specific adenosine deaminase

locus: BSU00180

pI: 5.64

mw: 17.61 kDa

protein length: 161 aa

gene length: 483 bp

function: tRNA modification

product: tRNA specific adenosine deaminase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 25,852 → 26,337

The protein

Protein family

• cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)

Structure

• [PDB|2B3J] (the protein of ''Staphylococcus aureus'', complex with RNA) [Pubmed|16415880]

Expression and Regulation

Operons

• genes: [gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]-[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, (both promoters), in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B891 (yaaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1890 NBRP B. subtilis, Japan]
• BKE00180 (Δ[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT, downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
• BKK00180 (Δ[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT, downstream forward: _UP4_TAGTACGGTTGCAATTTTTA

References

• 19087206,12110595,16415880