SubtiBank SubtiBank
yrkF
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yrkF

putative sulfur carrier protein
Locus
BSU_26530
Isoelectric point
5.99
Molecular weight
20.51 kDa
Protein length
185 aa Sequence Blast
Gene length
558 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,712,577 2,713,134

The protein

Protein family

  • sulfur carrier protein TusA family (with YrkI, according to UniProt)
  • Domains

  • Rhodanese domain (aa 101-185) (according to UniProt)
  • Structure

  • 1DCJ (E. coli TusA, corresponds to the N-terminal domain of YrkF, aa 8 ... 77, 37% identity)
  • 3R2U (metallo--lactamase from Staphylococcus aureus, corresponds to the C-terminal domain of YrkF, aa 106 ... 179, 38% identity)
  • Expression and Regulation

    Biological materials

    Mutant

  • BKE26530 (yrkF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTCCTCCTTTTCTT, downstream forward: _UP4_TAAATAAAAAGCATGATGAA
  • BKK26530 (yrkF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTCCTCCTTTTCTT, downstream forward: _UP4_TAAATAAAAAGCATGATGAA