ansB

L-aspartase

Locus: BSU_23570

Isoelectric point: 5.69

Molecular weight: 52.39 kDa

Protein length: 475 aa

Gene length: 1428 bp

Function: aspartate degradation

Product: L-aspartase

Essential: no

E.C.: 4.3.1.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of asparagine/ aspartate

Gene

Coordinates: 2,454,347 2,455,774

The protein

Catalyzed reaction/ biological activity

• L-aspartate --> fumarate + NH₄⁺ (according to UniProt)

Protein family

• class-II fumarase/aspartase family (with CitG, according to UniProt)

Paralogous protein(s)

• CitG

Structure

• 3R6Q (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed

Expression and Regulation

Operons

• Genes: ansA-ansB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon

  Regulation

  • expressed in the presence of asparagine (AnsR) PubMed

• Genes: ansA-ansB-mleN-mleA
  Description: PubMed

  Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed

Biological materials

Mutant

• GP1153 (ΔansA-ansB::ermC) available in Jörg Stülke's lab
• BKE23570 (ansB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
• BKK23570 (ansB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA

LacZ fusion

• pGP2274 (in pAC5) (GP2961), available in Jörg Stülke's lab

References

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