opuAB

glycine betaine and arsenobetaine ABC transporter (permease)

Locus: BSU_02990

Isoelectric point: 9.84

Molecular weight: 30.10 kDa

Protein length: 282 aa

Gene length: 849 bp

Function: compatible solute transport

Product: glycine betaine and arsenobetaine ABC transporter (permease)

Essential: no

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of compatible solutes for osmoprotection
• Lifestyles → Coping with stress → Coping with hyper-osmotic stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 322,271 323,119

The protein

Catalyzed reaction/ biological activity

• uptake of glycine betaine and arsenobetaine PubMed
• uptake of dimethylglycine PubMed

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• CysTW subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 90-269) (according to UniProt)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: opuAA-opuAB-opuAC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon

  Regulation

  • induced by osmotic stress PubMed

Biological materials

Mutant

• BKE02990 (opuAB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGTATTCTAGGCAGTCTAT, downstream forward: _UP4_AAGAAAAGCAGGGGGAATGC
• BKK02990 (opuAB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGTATTCTAGGCAGTCTAT, downstream forward: _UP4_AAGAAAAGCAGGGGGAATGC

Expression vectors

• pGP2925 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

Loading

Original publications

Loading