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queF [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine
function
tRNA modification
product
nitrile reductase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation][SW|Categories] containing this gene/protein
[SW|translation]This gene is a member of the following [SW|regulons]
[SW|preQ1 riboswitch]Gene
Coordinates on the chromosome (coding sequence)
1,441,291 -> 1,441,788
The protein
Catalyzed reaction/ biological activity
7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH (according to Swiss-Prot)Protein family
QueF type 1 subfamily (according to Swiss-Prot)Modification
active site Cys56 is S-bacillithiolated by NaOCl stress [Pubmed|22938038]Structure
[PDB|4F8B] (covalent thioimide intermediate of the unimodular nitrile reductase [protein|search|QueF]) [Pubmed|22787148][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operon
''[protein|search|queC]-[protein|search|queD]-[protein|search|queE]-[protein|search|queF]'' [Pubmed|14660578]Regulation
repressed in the presence of queuosine ([SW|preQ1 riboswitch]) [Pubmed|19285444]Regulatory mechanism
[SW|preQ1 riboswitch]: transcriptional antitermination in the absence of queuosine [Pubmed|19285444]References
22938038,14660578,15767583,16511203,17929836,19285444,17384645,22787148 
