queF [2017-03-21 16:18:21]

nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine

locus: BSU13750

pI: 4.93

mw: 19.00 kDa

protein length: 165 aa

gene length: 495 bp

function: tRNA modification

product: nitrile reductase

essential: no

ec: 1.7.1.13

synonyms: ykvM

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

This gene is a member of the following [SW|regulons]

• [SW|preQ1 riboswitch]

Gene

Coordinates: 1,441,291 -> 1,441,788

The protein

Catalyzed reaction/ biological activity

• 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH (according to Swiss-Prot)

Protein family

• QueF type 1 subfamily (according to Swiss-Prot)

Modification

• active site Cys56 is S-bacillithiolated by NaOCl stress [Pubmed|22938038]

Structure

• [PDB|4F8B] (covalent thioimide intermediate of the unimodular nitrile reductase [protein|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|QueF]) [Pubmed|22787148]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation: [[this]]

Biological materials

Mutant

• MGNA-B326 (ykvM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1325 NBRP B. subtilis, Japan]

References

• 22938038,14660578,15767583,16511203,17929836,19285444,17384645,22787148