queF

nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine

Locus: BSU_13750

Isoelectric point: 4.93

Molecular weight: 19.23 kDa

Protein length: 165 aa

Gene length: 498 bp

Function: tRNA modification

Product: nitrile reductase

Essential: no

E.C.: 1.7.1.13

Synonyms: ykvM

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 1,441,291 1,441,788

The protein

Catalyzed reaction/ biological activity

• 7-aminomethyl-7-carbaguanine + 2 NADP⁺ --> 7-cyano-7-deazaguanine + 3 H⁺ + 2 NADPH (according to UniProt)

Protein family

• GTP cyclohydrolase I family (with FolE, according to UniProt)

Modification

• active site Cys55 is S-bacillithiolated by NaOCl stress PubMed
• disulfide formation between the catalytic Cys55 and conserved Cys99 protects the protein from irreversible oxidation PubMed

Cofactors

• NADPH PubMed

Structure

• 4F8B (covalent thioimide intermediate of the unimodular nitrile reductase QueF) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: queC-queD-queE-queF
  Description: PubMed

  Regulatory mechanism

  • preQ1 riboswitch: antitermination, in the absence of queuosine PubMed, in preQ1 riboswitch

  Regulation

  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

Biological materials

Mutant

• MGNA-B326 (ykvM::erm), available at the NBRP B. subtilis, Japan
• BKE13750 (queF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTCATGTTCATCTTCCTTT, downstream forward: _UP4_TAATTATTCCGTTGTGTGAT
• BKK13750 (queF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTCATGTTCATCTTCCTTT, downstream forward: _UP4_TAATTATTCCGTTGTGTGAT

References

Loading