yngI [2019-04-16 12:28:01]

aceto-acetyl-CoA ligase, may be involved in surfactin production

locus: BSU18250

pI: 5.98

mw: 61.26 kDa

protein length: 549 aa

gene length: 1650 bp

function: mother cell metabolism, leucine utilization

product: aceto-acetyl-CoA ligase

essential: no

ec: 6.2.1.16

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 1,954,525 1,956,174

The protein

Catalyzed reaction/ biological activity

• ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA [Pubmed|19935659]

Paralogous protein(s)

• [protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|LcfA], [protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|LcfB], [protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|YhfT]

Structure

• [PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 32% identity) [pubmed|22560731]

Expression and Regulation

Operons

• genes: [gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
  description: [Pubmed|12662922]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]

Biological materials

Mutant

• MGNA-B087 (yngI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1086 NBRP B. subtilis, Japan]
• available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
• BKE18250 ([gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA
• BKK18250 ([gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA

References

• 15699190,12662922,19935659,20797616,22560731