SubtiBank SubtiBank
yngI
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yngI

aceto-acetyl-CoA ligase, may be involved in surfactin production
Locus
BSU_18250
Isoelectric point
5.98
Molecular weight
61.26 kDa
Protein length
549 aa Sequence Blast
Gene length
1650 bp Sequence Blast
Function
mother cell metabolism, leucine utilization
Product
aceto-acetyl-CoA ligase
Essential
no
E.C.
6.2.1.16
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    1,954,525 1,956,174

    The protein

    Catalyzed reaction/ biological activity

  • ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA PubMed

    • Protein family

  • ATP-dependent AMP-binding enzyme family (according to UniProt)

    • Paralogous protein(s)

  • LcfA, LcfB, YhfT

    • Structure

  • 3R44 (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 32% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    yngJ-yngI-yngH-yngHB-yngG-yngF-yngE
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed
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    Biological materials

    Mutant

  • MGNA-B087 (yngI::erm), available at the NBRP B. subtilis, Japan
  • available in Mohamed Marahiel's lab PubMed
  • BKE18250 (yngI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA
  • BKK18250 (yngI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA

    • References

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