ybdJ

two-component response regulator ([SW|OmpR family])

locus: BSU_02000

pI: 6.35

mw: 26.03 kDa

protein length: 223 aa

gene length: 672 bp

function: unknown

product: two-component response regulator ([SW|OmpR family])

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 221,258 221,929

The protein

Protein family

• [SW|OmpR family] of two-component response regulators

[SW|Domains]

• [SW|Response regulatory domain] (aa 5-116) (according to UniProt)

Modification

• phosphorylated by [protein|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|YbdK] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|1KGS](from Thermotoga maritima, 32% identity) [pubmed|11839301]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-B956 (ybdJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1955 NBRP B. subtilis, Japan]
• BKE02000 ([gene|5F6D14A0B360832F9FD470668B006A628B109A56|ybdJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAAAATACGATAACCCT, downstream forward: _UP4_TGAAGCTCAAGACAAAATAT
• BKK02000 ([gene|5F6D14A0B360832F9FD470668B006A628B109A56|ybdJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAAAATACGATAACCCT, downstream forward: _UP4_TGAAGCTCAAGACAAAATAT

References

• 10094672,23504016,11839301