rpoY [2019-02-19 19:22:23]

epsilon subunit of RNA polymerase

locus: BSU14540

pI: 4.61

mw: 8.12 kDa

protein length: 69 aa

gene length: 210 bp

function: control of RNA polymerase activity

product: epsilon subunit of RNA polymerase

essential: no

synonyms: ykzG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]

Gene

Coordinates: 1,524,791 1,525,000

The protein

Protein family

• UPF0356 family (according to Swiss-Prot)

Structure

• [PDB|4NJC] [Pubmed|25092033]

[SW|Localization]

• colocalizes with the nucleoid [Pubmed|25092033]

Expression and Regulation

Operons

• genes: [gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]-[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]
  description: [Pubmed|24187087]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|24187087], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE14540 ([gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA, downstream forward: _UP4_AAAGTATTGGAGTTATGAGC
• BKK14540 ([gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA, downstream forward: _UP4_AAAGTATTGGAGTTATGAGC

Expression vectors

• pGP2824: expression in ''B. subtilis'', with C-terminal Strep-tag, in [SW|pGP382], available in [SW|Jrg Stlke]' lab

lacZ fusion

• pGP420 (in [protein|search|pAC7]), available in [SW|Stlke] lab

References

Reviews

• 25878038

Original publications

• 24187087,20724389,25092033