yloN [2019-01-30 12:33:16]

similar to rRNA adenosine methyltransferase for modification of 23S rRNA

locus: BSU15750

pI: 6.79

mw: 41.41 kDa

protein length: 363 aa

gene length: 1092 bp

function: rRNA modification

product: rRNA methyltransferase

essential: no

synonyms: rlmN

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 1,649,286 1,650,377

The protein

Catalyzed reaction/ biological activity

• methylates C-2 in A2503 in 23S rRNA and A37 in tRNA [pubmed|23752511]

Protein family

• RlmN family (according to Swiss-Prot), SAM enzyme superfamily

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|3RF9] (from E. coli, 38% identity) [pubmed|21527678]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
  description: [Pubmed|16964327]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16964327], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

Biological materials

Mutant

• MGNA-B132 (yloN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1131 NBRP B. subtilis, Japan]
• 1A819 ( ''yloN''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A819&Search=1A819 BGSC]
• BKE15750 ([gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA, downstream forward: _UP4_CAAGACGAGACGAGGTGATG
• BKK15750 ([gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA, downstream forward: _UP4_CAAGACGAGACGAGGTGATG

References

• 18025251,18307109,27496281,23752511,11918677,21527678