inhibitor of motility and glycosyltransferase required for EPS biosynthesis
function
biofilm formation
product
glycosyltransferase, inhibitor of motility
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue][SW|Categories] containing this gene/protein
[SW|motility and chemotaxis], [SW|biofilm formation], [SW|membrane proteins], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|AbrB regulon], [SW|EAR riboswitch], [SW|RemA regulon], [SW|SinR regulon]Gene
Coordinates on the chromosome (coding sequence)
3,524,417 -> 3,525,253
Phenotypes of a mutant
smooth colonies on MsGG medium, no [SW|biofilm formation] [Pubmed|22113911]The protein
Catalyzed reaction/ biological activity
arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix, separates the cytoplasmic [protein|search|FliG] motor from the [protein|search|MotA]-[protein|search|MotB] stator [Pubmed|18566286]biosynthesis of extracellular polysaccharides [Pubmed|21170308]Protein family
glycosyltransferase 2 family (according to Swiss-Prot)Modification
phosphorylated by [protein|search|EpsB] on a Tyr residue [Pubmed|25085422][SW|Localization]
cell membrane, forms spots at flagellar basal bodies [Pubmed|18566286][SW|Interactions]
[protein|search|EpsE]-[protein|search|FliG] [Pubmed|18566286]Expression and Regulation
Operon
''[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]'' [Pubmed|15661000][SW|Sigma factor]
[protein|search|SigA] [Pubmed|15661000]Regulation
repressed by [protein|search|SinR] [Pubmed|15661000]Regulatory mechanism
[protein|search|SinR]: transcription anti-activation (prevents binding of [protein|search|RemA]) [Pubmed|23646920][protein|search|RemA]: transcription activation [Pubmed|23646920][protein|search|AbrB]: transcription repression [Pubmed|20817675]Additional information
induction by sequestration of [protein|search|SinR] by [protein|search|SinI] or [protein|search|SlrA] [PubMed|15661000,19788541]the '[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]' operon is not expressed in a '[protein|search|ymdB]' mutant [PubMed|21856853]the amount of the mRNA is substantially decreased upon depletion of [Rny|search|RNase Y] (this is likely due to the increased stability of the '[protein|search|sinR]' mRNA) [PubMed|21815947]the [[EAR riboswitch]] (eps-associated [[RNA switch]]) located between' [protein|search|epsB]' and '[protein|search|epsC]' mediates processive antitermination and allows expression of the long eps operon [PubMed|20374491]Labs working on this gene/protein
[SW|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage][SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]References
Reviews
20735481,24988880,25251856 =The EAR [SW|RNA switch]=
