fabF

beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity

Locus: BSU_11340

Isoelectric point: 4.77

Molecular weight: 43.84 kDa

Protein length: 413 aa

Gene length: 1242 bp

Function: fatty acid biosynthesis

Product: beta-ketoacyl-acyl carrier protein synthase II

Essential: yes

E.C.: 2.3.1.179

Synonyms: yjaY

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Membrane dynamics
• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of fatty acids
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Essential genes

Gene

Coordinates: 1,209,183 1,210,424

Phenotypes of a mutant

• essential PubMed
• CRISPRi knock-down: terminal phenotype of growth cessation, moderate bending PubMed
• FabF-I109F: resistance to cerulenin PubMed
• FabF-L112F, FabF-I109M: resistance to cerulenin (Zeigler DR, unpublished observations)

The protein

Catalyzed reaction/ biological activity

• (11Z)-hexadecenoyl-[ACP] + H⁺ + malonyl-[ACP] --> 3-oxo-(13Z)-octadecenoyl-[ACP] + CO₂ + holo-[ACP](according to UniProt)
• SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed

Protein family

• thiolase-like superfamily (according to UniProt)

Structure

• 4LS5 PubMed

Expression and Regulation

Operons

• Genes: fabHA-fabF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigW: sigma factor, PubMed, in SigW regulon

  Regulatory mechanism

  • FapR: repression, PubMed, in FapR regulon

  Regulation

  • fabHA: expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • expression of the operon is strongly reduced during biofilm formation PubMed

Biological materials

Mutant

• BEC11340 (lacA::Pxyl-dcas9 amyE::Pveg-sgRNA(fabF) trpC2) BGSC, PubMed
• 1A573 (fabF2 trpC2) BGSC, cerulenin-resistant mutant
• 1A576 (fabF14 trpC2) BGSC, cerulenin-resistant mutant
• 1A577 (fabF20 trpC2) BGSC, cerulenin-resistant mutant

Expression vector

• pGP3509 (N-terminal His-tag, expression in E. coli, in pWH844), available in Jörg Stülke's lab
• pGP3510: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pETSUMOadapt, available in Jörg Stülke's lab
• pGP3511: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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