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pyrF [2018-06-29 17:26:34]
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pyrF [2018-06-29 17:26:34]

orotidine 5-phosphate decarboxylase
Locus
BSU15550
Isoelectric point
5.84
Molecular weight
25.84 kDa
Protein length
239 aa Sequence Blast
Gene length
717 bp Sequence Blast
Function
pyrimidine biosynthesis
Product
orotidine-5-phosphate decarboxylase
Essential
no
E.C.
4.1.1.23
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,628,622 → 1,629,341

The protein

Catalyzed reaction/ biological activity

  • Orotidine 5'-phosphate = UMP + CO2 (according to Swiss-Prot)
  • Protein family

  • Type 1 subfamily (according to Swiss-Prot)
  • Structure

    Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon
  • Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed
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    Biological materials

    Mutant

  • BKE15550 (ΔpyrF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAGTGTTTCTTCAGCTGACG, downstream forward: _UP4_GCCTATAAGGCTGTCAGACT
  • BKK15550 (ΔpyrF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAGTGTTTCTTCAGCTGACG, downstream forward: _UP4_GCCTATAAGGCTGTCAGACT
  • References

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