dnaC

replicative DNA helicase, part of the [SW|replisome]

locus: BSU_40440

pI: 4.55

mw: 50.44 kDa

protein length: 454 aa

gene length: 1365 bp

function: [category|SW 3.1.1|DNA replication]

product: replicative DNA helicase

essential: yes

ec: 3.6.4.12

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 4,157,471 4,158,835

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• stimulates [protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] primase activity [Pubmed|23563155]
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• [SW|helicase family] (according to UniProt)

[SW|Domains]

• SF4 helicase domain (aa 179-445) (according to UniProt)

Structure

• [PDB|2R6A] (Geobacillus stearothermophilus), [PDB|2R6C], [PDB|2R6D], [PDB|2R6E]
• [PDB|4M4W] (the [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]-[protein|AC1B507C6B1640E9BEA1F9F01628120F3680C976|DnaI]-[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] complex) [Pubmed|24048025]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC]
  description: [pubmed|22383849]

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

References

Reviews

• 22797751,28075389

Original publications

• 10844689,12718886,11250911,20122408,16479537,10844689,19744498,19968790,12060778,23525462,23563155,24048025,30285297