rluB

ribosomal large subunit pseudouridine synthase B

locus: BSU_23160

pI: 10.25

mw: 25.88 kDa

protein length: 244 aa

gene length: 735 bp

function: 23S rRNA modification

product: pseudouridine synthase

essential: no

ec: 5.4.99.22

synonyms: ypuL

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 2,421,438 2,422,172

The protein

Catalyzed reaction/ biological activity

• uridine²⁶⁰⁵ in 23S rRNA a pseudouridine²⁶⁰⁵ in 23S rRNA (according to E. coli RluB)

Protein family

• [SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RsuA family (with [protein|69052B24B577ABA4B20E610F679154DC97EFD8DE|YtzG], according to UniProt)

[SW|Domains]

• [SW|S4 RNA-binding domain] (aa 2-68) (according to UniProt)

Structure

• [PDB|4LAB] (from ''E. coli'', 40% identity) [Pubmed|24214967]

Expression and Regulation

Operons

• genes: [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A106 (ypuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/106 NBRP B. subtilis, Japan]
• BKE23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA
• BKK23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA

References

• 9888802,24214967