SubtiBank SubtiBank
rluB
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rluB

ribosomal large subunit pseudouridine synthase B
Locus
BSU_23160
Isoelectric point
10.25
Molecular weight
25.88 kDa
Protein length
244 aa Sequence Blast
Gene length
735 bp Sequence Blast
Function
23S rRNA modification
Product
pseudouridine synthase
Essential
no
E.C.
5.4.99.22
Synonyms
ypuL

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,421,438 2,422,172

The protein

Catalyzed reaction/ biological activity

  • uridine2605 in 23S rRNA a pseudouridine2605 in 23S rRNA (according to E. coli RluB)
  • Protein family

  • S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
  • pseudouridine synthase RsuA family (with YtzG, according to UniProt)
  • Domains

  • S4 RNA-binding domain (aa 2-68) (according to UniProt)
  • Structure

  • 4LAB (from E. coli, 40% identity) PubMed
  • Expression and Regulation

    Biological materials

    Mutant

  • MGNA-A106 (ypuL::erm), available at the NBRP B. subtilis, Japan
  • BKE23160 (rluB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA
  • BKK23160 (rluB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA
  • References

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