yusV

[SW|ABC transporter] for the siderophores Fe-enterobactin and Fe-bacillibactin, as well as for the siderophores schizokinen and arthrobactin (ATPase)

locus: BSU_32940

pI: 5.32

mw: 30.34 kDa

protein length: 275 aa

gene length: 828 bp

function: acquisition of iron

product: [SW|ABC transporter] for the siderophores Fe-enterobactin, Fe-bacillibactin, schizokinen and arthrobactin (ATPase)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,379,112 3,379,939

The protein

Catalyzed reaction/ biological activity

• ATPase for the [protein|516B13F337FD346B4A4A268E35D1EBABB05957E1|FeuA]-[protein|57402D137E3D3791CAF0696421224F4E1DC1BA48|FeuB]-[protein|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|FeuC] siderophore [SW|ABC transporter] and for the [protein|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|YfhA]-[protein|521246EDA87A00F1B5E0792EC7AF1EF2B11C1C3C|YfiY]-[protein|81494D4E7E52531802C3C0D0073739DA9375F5DF|YfiZ] siderophore [SW|ABC transporter]

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|E70BE74D5AB4B06CDFF902E58A0B9EBF477E21EC|FhuC], [protein|E76640F895261DA34AD2342B54B43D11857AEA9A|FecF], [protein|473628F86C18FE9957841F3C8B45242C90CB341D|FpbP]

[SW|Domains]

• [SW|ABC transporter domain] (aa 6-242) (according to UniProt)

Structure

• [PDB|4R9U], the ''E. coli'' BtuC-BtuD complex, BtuD shares 32% identity, 57% similarity with YusV, [Pubmed|25402482]

[SW|Localization]

• associated to the membrane (via [protein|57402D137E3D3791CAF0696421224F4E1DC1BA48|FeuB]-[protein|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|FeuC] and [protein|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|YfhA]-[protein|81494D4E7E52531802C3C0D0073739DA9375F5DF|YfiZ]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV]-[gene|FCA17A66EFD82BC999E3F062FB6ECCA0B413E3FB|yusU]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

Biological materials

Mutant

• MGNA-B599 (yusV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1598 NBRP B. subtilis, Japan]
• BKE32940 ([gene|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACCGCTCCTTTTTGCT, downstream forward: _UP4_TAAATAACGCAGCGAAGGAG
• BKK32940 ([gene|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACCGCTCCTTTTTGCT, downstream forward: _UP4_TAAATAACGCAGCGAAGGAG

References

• 19746494,10092453,16672620,12354229,25402482