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cadA
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cadA

cadmium transporting ATPase, resistance to cadmium
Locus
BSU_33490
Isoelectric point
6.00
Molecular weight
75.22 kDa
Protein length
699 aa Sequence Blast
Gene length
2100 bp Sequence Blast
Function
cadmium export
Product
cadmium transporting ATPase
Essential
no
E.C.
3.6.3.3
Synonyms
yvgW

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,438,853 3,440,952

The protein

Catalyzed reaction/ biological activity

  • ATP + Cd2+ + H2O --> ADP + Cd2+ + H+ + phosphate (according to UniProt)
  • ATP + H2O + Zn2+ --> ADP + H+ + phosphate + Zn2+ (according to UniProt)
  • Protein family

    Paralogous protein(s)

    Domains

  • HMA domain (aa 5-73) (according to UniProt)
  • Structure

  • 4BBJ (CopA from Legionella pneumophila, 32% identity, 67% similarity) PubMed
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CzrA: repression, PubMed, in CzrA regulon
  • Regulation

  • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed
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    Biological materials

    Mutant

  • MGNA-A491 (yvgW::erm), available at the NBRP B. subtilis, Japan
  • BKE33490 (cadA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTAGTCTCACCTTACCTT, downstream forward: _UP4_TAAATTGTCGGAGAGAATTC
  • BKK33490 (cadA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTAGTCTCACCTTACCTT, downstream forward: _UP4_TAAATTGTCGGAGAGAATTC
  • Labs working on this gene/protein

  • John Helmann, Cornell University, USA Homepage
  • References

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