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pbpC [2018-02-16 18:28:51]
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pbpC [2018-02-16 18:28:51]

transpeptidase, penicillin-binding protein 3
Locus
BSU04140
Isoelectric point
6.20
Molecular weight
74.23 kDa
Protein length
668 aa Sequence Blast
Gene length
2004 bp Sequence Blast
Function
unknown
Product
penicillin-binding protein 3
Essential
no
Synonyms
ycsM,yzsA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
  • Groups of genes → Secreted proteins

    • Gene

    Coordinates
    463,934 → 465,940

    Phenotypes of a mutant

  • increased sensitivity to several β-lactams PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • cross-linking of glycan chains PubMed

    • Protein family

  • transpeptidase family (according to Swiss-Prot)

    • Structure

  • 5E31 (from Enterococcus faecium, 40% identity)

    • Localization

  • cell membrane (according to Swiss-Prot)
  • mid cell localization (divisome), this depends on FtsZ and PBP2A PubMed
  • extracellular (signal peptide) PubMed

    • Expression and Regulation

    Operons

    Genes
    pbpC-ycsN
    Description
    PubMed
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    Biological materials

    Mutant

  • BKE04140 (ΔpbpC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
  • BKK04140 (ΔpbpC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG

    • GFP fusion

  • 3105 trpC2 pbpC::pSG5045 (cat Pxyl-gfpa-pbpC1-768) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

    • References

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