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pbpC [2020-05-28 15:24:25]
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pbpC [2020-05-28 15:24:25]

transpeptidase, penicillin-binding protein 3
locus
BSU_04140
pI
6.20
mw
74.23 kDa
protein length
668 aa Sequence Blast
gene length
2007 bp Sequence Blast
function
unknown
product
penicillin-binding protein 3
essential
no
synonyms
ycsM,yzsA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    463,934 465,940

    Phenotypes of a mutant

  • increased sensitivity to several -lactams [pubmed|28792086]

    • The protein

    Catalyzed reaction/ biological activity

  • cross-linking of glycan chains [pubmed|28792086]

    • Protein family

  • [SW|transpeptidase family] (according to UniProt)

    • Structure

  • [PDB|5E31] (from Enterococcus faecium, 40% identity)

    • [SW|Localization]

  • mid cell localization (divisome), this depends on [protein|search|FtsZ ]and [protein|EBEFF9E0A524DCDA19382E5401B923B805E4C559|PBP2A ][pubmed|28792086]
  • extracellular (signal peptide) [Pubmed|18957862]
  • during vegetative growth: distinct foci and bands at cell periphery [pubmed|14731276]
  • localizes as patches in single cells and concentrates as a ring at the septum and the division site during the cell growth cycle [pubmed|32155044]

    • Expression and Regulation

    Operons

    genes
    [gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]-[gene|D0A2506FDCEA41D2A8910AC2837B46E0B311D63B|ycsN]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • BKE04140 ([gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
  • BKK04140 ([gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG

    • GFP fusion

  • 3105 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::pSG5045 (cat Pxyl-gfpa-[gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]1-768) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • References

  • 8830698,14731276,20487272,18957862,28792086,32155044