cysE [2019-06-06 17:00:33]

serine O-acetyltransferase

Locus: BSU00930

Isoelectric point: 9.49

Molecular weight: 24.00 kDa

Protein length: 217 aa

Gene length: 654 bp

Function: biosynthesis of cysteine

Product: serine O-acetyltransferase

Essential: no

E.C.: 2.3.1.30

Synonyms: cysA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
• Information processing → Genetics → Newly identified competence genes

Gene

Coordinates: 112,800 113,453

Phenotypes of a mutant

• poor growth PubMed
• non-transformable PubMed

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine (according to Swiss-Prot)

Protein family

• Transferase hexapeptide repeat family (according to UniProt)

Structure

• 1T3D (from Escherichia coli, 48% identity, 64% similarity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: gltX-cysE-cysS-mrnC-yacO-yacP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: transcription antitermination, overlaps a transcription terminator upstream of cysE, in T-box

  Regulation

  • expression transiently increases in the forespore PubMed
  • the T-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• BKE00930 (cysE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATGCTTCCCCCCGTTTC, downstream forward: _UP4_AGAAAAGAAAGGATCAATCA
• BKK00930 (cysE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATGCTTCCCCCCGTTTC, downstream forward: _UP4_AGAAAAGAAAGGATCAATCA

References

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