cysE

serine O-acetyltransferase

locus: BSU_00930

pI: 9.49

mw: 24.00 kDa

protein length: 217 aa

gene length: 654 bp

function: biosynthesis of cysteine

product: serine O-acetyltransferase

essential: no

ec: 2.3.1.30

synonyms: cysA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]

Gene

Coordinates: 112,800 113,453

Phenotypes of a mutant

• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + L-serine --> CoA + O-acetyl-L-serine (according to UniProt)

Protein family

• [SW|Transferase hexapeptide repeat family] (according to UniProt)

Structure

• [PDB|1T3D] (from ''Escherichia coli'', 48% identity, 64% similarity) [Pubmed|15231846]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]
  description: [Pubmed|7510287]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7510287], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|T-box|T-box]

  regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• BKE00930 ([gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATGCTTCCCCCCGTTTC, downstream forward: _UP4_AGAAAAGAAAGGATCAATCA
• BKK00930 ([gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATGCTTCCCCCCGTTTC, downstream forward: _UP4_AGAAAAGAAAGGATCAATCA

References

• 19258532,15231846,7510287,28189581