tmk [Fri Jun 10 2016 15:33:46 GMT+0200 (CEST)]

thymidylate kinase

locus: BSU00280

pI: 4.00

mw: 23.00 kDa

function: TTP biosynthesis

product: thymidylate kinase

essential: yes

ec: 2.7.4.9

synonyms: yaaP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes]

Gene

Coordinates on the chromosome (coding sequence): 39,159 -> 39,797

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP dTMP = ADP dTDP (according to Swiss-Prot)

Protein family

• thymidylate kinase family (according to Swiss-Prot)

Structure

• [PDB|2CCK] (from ''Staphylococcus aureus'', 53% identity, 74% similarity) [Pubmed|16522804]

Biological materials

Expression vector

• pGP1689: expression of Strep-''tmk'' in ''E. coli'', in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
• pGP2671: expression of ''tmk''-Strep, in [SW|pGP380] ,in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP2401 ''tmk-3xFLAG spc'' (based on [SW|pGP2786]), available in [SW|Jörg Stülke]'s lab

References

• 16522804,18523102,23043329