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ycbD [2019-07-22 10:00:44]
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ycbD [2019-07-22 10:00:44]

NADP+-dependent alpha-ketoglutaric semialdehyde dehydrogenase
Locus
BSU_02470
Isoelectric point
4.79
Molecular weight
52.25 kDa
Protein length
488 aa Sequence Blast
Gene length
1467 bp Sequence Blast
Function
utilization of D-glucarate/galactarate
Product
NADP+-dependent alpha-ketoglutaric semialdehyde dehydrogenase
Essential
no
E.C.
1.2.1.3
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glucarate/galactarate

    • Gene

    Coordinates
    268,846 270,312

    The protein

    Catalyzed reaction/ biological activity

  • an aldehyde + NAD+ + H2O = an acid + NADH (according to Swiss-Prot)

    • Protein family

  • aldehyde dehydrogenase family (according to UniProt)

    • Paralogous protein(s)

  • DhaS, RocA, IolA, YwdH, GabD, YfmT, AldX, AldY, GbsA, PutC

    • Cofactors

  • NAD+

    • Structure

  • 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 36% identity, 69% similarity)

    • Expression and Regulation

    Operons

    Genes
    ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ
    Description
    PubMed

    Regulatory mechanism

  • YcbG: repression, PubMed, in YcbG regulon
  • AbrB: repression, PubMed, in AbrB regulon

    • Regulation

  • induced by D-glucarate/galactarate (YcbG) PubMed
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    Biological materials

    Mutant

  • BKE02470 (ycbD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCCATTGCCCCTTTCG, downstream forward: _UP4_TAATGGTGCTGGAAAGAGGC
  • BKK02470 (ycbD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCCATTGCCCCTTTCG, downstream forward: _UP4_TAATGGTGCTGGAAAGAGGC

    • References

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