yfkC [2018-11-01 18:04:58]

mechanosensitive channel, similar to MscS

locus: BSU07940

pI: 5.29

mw: 32.05 kDa

protein length: 280 aa

gene length: 840 bp

function: resistance to osmotic downshock

product: mechanosensitive channel, similar to MscS

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.7|Coping with hypo-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 867,164 → 868,006

The protein

Structure

• [PDB|3UDC] (36% identity) [pubmed|23074248]

[SW|Localization]

• cell membrane [Pubmed|19252899]

Expression and Regulation

Operons

• genes: [gene|62718BBECEB18244B5CC877509679F5F9A0C50CD|yfkA]-[gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C271 (yfkC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2269 NBRP B. subtilis, Japan]
• 1A959 ( ''yfkC''::''tet''), [Pubmed|18310427], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A959&Search=1A959 BGSC]
• BKE07940 (Δ[gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTGTTTCTTTCATCC, downstream forward: _UP4_TAAACAAAAAAACTGATTCC
• BKK07940 (Δ[gene|5ACE71EBBDCEB0C534A91DE303FD8069BEFDD6A1|yfkC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTGTTTCTTTCATCC, downstream forward: _UP4_TAAACAAAAAAACTGATTCC

Labs working on this gene/protein

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 12626684,22685280,22404681,24607989,17505523

Original Publications

• 17665170,18310427,19252899,22383849,23074248