uppP

minor undecaprenyl pyrophosphate phosphatase

Locus: BSU_31150

Isoelectric point: 7.90

Molecular weight: 30.19 kDa

Protein length: 276 aa

Gene length: 831 bp

Function: synthesis of undecaprenyl phosphate as precursor for lipid II

Product: minor undecaprenyl pyrophosphate phosphatase

Essential: no

E.C.: 3.6.1.27

Synonyms: yubB

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Membrane proteins
• Groups of genes → Poorly characterized/ putative enzymes

Gene

Coordinates: 3,194,635 3,195,465

Phenotypes of a mutant

• inactivation of uppP reduces sporulation efficiency to 0.4% that of wild type cells; distended intermembrane space PubMed
• a bcrC uppP double mutant is not viable (unless pgpB is artificially expressed) PubMed

The protein

Catalyzed reaction/ biological activity

• di-trans,octa-cis-undecaprenyl diphosphate + H₂O --> di-trans,octa-cis-undecaprenyl phosphate + H⁺ + phosphate (according to UniProt)

Protein family

• uppP family (single member, according to UniProt)

Structure

• 5OON (from E. coli, 47% identity) PubMed

Localization

• membrane associated PubMed

Additional information

• UppP seems to be a minor undecaprenyl pyrophosphate phosphatase, the major enzyme is BcrC PubMed

Expression and Regulation

Operons

• Genes: yubA-uppP
  Description: PubMed

Biological materials

Mutant

• MGNA-A224 (yubB::erm), available at the NBRP B. subtilis, Japan
• BKE31150 (uppP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTTGATTCTCCCCA, downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC
• BKK31150 (uppP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTTGATTCTCCCCA, downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC

References

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