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sinR [Tue Jan 03 2017 18:01:02 GMT+0100 (CET)]
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sinR [Tue Jan 03 2017 18:01:02 GMT+0100 (CET)]

transcriptional regulator (Xre family) of post-exponential-phase responses genes
locus
BSU24610
pI
7.00
mw
12.00 kDa
function
control of biofilm formation
product
transcriptional regulator (Xre family)
essential
no
synonyms
sin, flaD

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]
  • [SW|Categories] containing this gene/protein

  • [SW|transcription factors and their control], [SW|transition state regulators], [SW|biofilm formation]
  • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|ScoC regulon], [SW|Spo0A regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,552,653 -> 2,552,988

    Phenotypes of a mutant

  • the mutation suppresses the galactose toxicity to a'' [protein|search|galE]'' mutant [Pubmed|22893383]
  • The protein

    Catalyzed reaction/ biological activity

  • transcription regulator of biofilm genes, acts as a true repressor of the ''[protein|search|tapA]-[protein|search|sipW]-[protein|search|tasA]'' operon and as an anti-activator (prevents binding of the activator protein [protein|search|RemA]) of the ''[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]'' operon [Pubmed|23646920]
  • acts as co-repressor for [protein|search|SlrR] [Pubmed|20351052]
  • Protein family

  • [SW|Xre family]
  • Paralogous protein(s)

  • [protein|search|SlrR]
  • [SW|Domains]

  • DNA-binding N-terminal domain (aa 1-69) [Pubmed|21708175]
  • [protein|search|SinI]-binding C-terminal domain (aa 74-111) [Pubmed|21708175]
  • Effectors of protein activity

  • [protein|search|SinI] and [protein|search|SlrA] are antagonists to [protein|search|SinR] [Pubmed|19788541]
  • [protein|search|SlrR] is also antagonist of [protein|search|SinR] [Pubmed|20351052]
  • Structure

  • [PDB|2YAL] (C-terminal domain, aa 74-111) [Pubmed|21708175]
  • [PDB|3QQ6] (N-terminal domain, aa 1-69) [Pubmed|21708175]
  • [PDB|1B0N] (complex [protein|search|SinR]-[protein|search|SinI]) [Pubmed|9799632]
  • [SW|Interactions]

  • [protein|search|SlrA]-[protein|search|SinR] [Pubmed|19788541], K(D) 10.6 nM [Pubmed|23430750]
  • [protein|search|SlrR]-[protein|search|SinR] [Pubmed|20351052], K(D) 47.5 nM [Pubmed|23430750], [protein|search|Veg] may inhibit this interaction [Pubmed|23378512]
  • [protein|search|SinR]-[protein|search|SinI] [Pubmed|9799632], K(D) 1.8 nM [Pubmed|23430750]
  • [protein|search|SinR]-[protein|search|ScoC]
  • Expression and Regulation

    Operon

  • ''[protein|search|sinI]-[protein|search|sinR]'' [Pubmed|3125149]
  • ''[protein|search|sinR]'' [Pubmed|3125149]
  • [SW|Sigma factor]

  • ''[protein|search|sinI]'': [protein|search|SigA] [Pubmed|3125149]
  • ''[protein|search|sinR]'': [protein|search|SigA] [Pubmed|3125149]
  • Regulation

  • ''[protein|search|sinI]'': repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|11751836]
  • ''[protein|search|sinI]'': repressed during exponential growth ([protein|search|ScoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
  • ''[protein|search|sinI]'': repressed during logrithmic growth ([protein|search|AbrB]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
  • ''[protein|search|sinI]'': repressed during vegetative growth ([protein|search|SinR]) [Pubmed|1664536]
  • ''[protein|search|sinR]'' is bimodally expressed and this depends on [rny|search|RNase Y] [Pubmed|26819068]
  • Regulatory mechanism

  • [SW|Spo0A]: transcription repression [Pubmed|11751836]
  • [protein|search|ScoC]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
  • [protein|search|AbrB]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
  • [protein|search|SinR]: transcription repression [Pubmed|1664536]
  • Additional information

  • the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] (the half-life of the mRNA increases from 3.5 to 13 min) [PubMed|21815947]
  • RNA degradation by [Rny|search|RNase Y] was reported to depend on the interaction of [Rny|search|RNase Y] with the complex of [protein|search|YmcA]-[protein|search|YlbF]-[protein|search|YaaT] [Pubmed|26434553]
  • Biological materials

    Mutant

  • GP923 (''sinR::spec'') [Pubmed|21856853], available in [SW|Jörg Stülke]'s lab
  • GP736 (''sinR::tetR'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
  • 1S97 (''sinR''::''phleo''), [Pubmed|8422983], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]
  • GP1672 (''[protein|search|sinR]-[protein|search|tasA]''::''cat'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
  • GP1663 (''[protein|search|yqhG]-[protein|search|sinI]-[protein|search|sinR]-[protein|search|tasA]''), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]: pGP1083 , available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • pGP1930 (aphA3) based on [protein|search|pAC7], available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP960 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 20395361,20541494,23353768,24988880
  • Modelling of the [protein|search|SinI]/[protein|search|SinR] switch

  • 21095906
  • Original publications

  • 22893383,23378512,23430750,23475644,21856853,21815947,22329926,21326214,21708175,8955328,15661000,8878039,24317403,16923912,15104138,16430695,16430696,18047568,18430133,1906467,7635837,11751836,19201793,10547280,15104138,9799632,19788541,19898538,3125149,8932324,20351052,20923420,8422983,9685500,9158733,23646920,23660663,24256735,24347549,25433524,26283769,26819068,26434553