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sinR [Wed Mar 09 2016 16:15:47 GMT+0100 (CET)]
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sinR [Wed Mar 09 2016 16:15:47 GMT+0100 (CET)]

transcriptional regulator (Xre family) of post-exponential-phase responses genes
locus
BSU24610
pI
7.00
mw
12.00 kDa
function
control of biofilm formation
product
transcriptional regulator (Xre family)
essential
no
synonyms
sin, flaD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]

    • [SW|Categories] containing this gene/protein

  • [SW|transcription factors and their control], [SW|transition state regulators], [SW|biofilm formation]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|ScoC regulon], [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,552,653 -> 2,552,988

    Phenotypes of a mutant

  • the mutation suppresses the galactose toxicity to a'' [protein|search|galE]'' mutant [Pubmed|22893383]

    • The protein

    Catalyzed reaction/ biological activity

  • transcription regulator of biofilm genes, acts as a true repressor of the ''[protein|search|tapA]-[protein|search|sipW]-[protein|search|tasA]'' operon and as an anti-activator (prevents binding of the activator protein [protein|search|RemA]) of the ''[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]'' operon [Pubmed|23646920]
  • acts as co-repressor for [protein|search|SlrR] [Pubmed|20351052]

    • Protein family

  • [SW|Xre family]

    • Paralogous protein(s)

  • [protein|search|SlrR]

    • [SW|Domains]

  • DNA-binding N-terminal domain (aa 1-69) [Pubmed|21708175]
  • [protein|search|SinI]-binding C-terminal domain (aa 74-111) [Pubmed|21708175]

    • Effectors of protein activity

  • [protein|search|SinI] and [protein|search|SlrA] are antagonists to [protein|search|SinR] [Pubmed|19788541]
  • [protein|search|SlrR] is also antagonist of [protein|search|SinR] [Pubmed|20351052]

    • Structure

  • [PDB|2YAL] (C-terminal domain, aa 74-111) [Pubmed|21708175]
  • [PDB|3QQ6] (N-terminal domain, aa 1-69) [Pubmed|21708175]
  • [PDB|1B0N] (complex [protein|search|SinR]-[protein|search|SinI]) [Pubmed|9799632]

    • [SW|Interactions]

  • [protein|search|SlrA]-[protein|search|SinR] [Pubmed|19788541], K(D) 10.6 nM [Pubmed|23430750]
  • [protein|search|SlrR]-[protein|search|SinR] [Pubmed|20351052], K(D) 47.5 nM [Pubmed|23430750], [protein|search|Veg] may inhibit this interaction [Pubmed|23378512]
  • [protein|search|SinR]-[protein|search|SinI] [Pubmed|9799632], K(D) 1.8 nM [Pubmed|23430750]
  • [protein|search|SinR]-[protein|search|ScoC]

    • Expression and Regulation

    Operon

  • ''[protein|search|sinI]-[protein|search|sinR]'' [Pubmed|3125149]
  • ''[protein|search|sinR]'' [Pubmed|3125149]

    • [SW|Sigma factor]

  • ''[protein|search|sinI]'': [protein|search|SigA] [Pubmed|3125149]
  • ''[protein|search|sinR]'': [protein|search|SigA] [Pubmed|3125149]

    • Regulation

  • ''[protein|search|sinI]'': repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|11751836]
  • ''[protein|search|sinI]'': repressed during exponential growth ([protein|search|ScoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
  • ''[protein|search|sinI]'': repressed during logrithmic growth ([protein|search|AbrB]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
  • ''[protein|search|sinI]'': repressed during vegetative growth ([protein|search|SinR]) [Pubmed|1664536]
  • ''[protein|search|sinR]'' is bimodally expressed and this depends on [rny|search|RNase Y] [Pubmed|26819068]

    • Regulatory mechanism

  • [SW|Spo0A]: transcription repression [Pubmed|11751836]
  • [protein|search|ScoC]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
  • [protein|search|AbrB]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
  • [protein|search|SinR]: transcription repression [Pubmed|1664536]

    • Additional information

  • the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] (the half-life of the mRNA increases from 3.5 to 13 min) [PubMed|21815947]

    • Biological materials

    Mutant

  • GP923 (''sinR::spec'') [Pubmed|21856853], available in [SW|Jörg Stülke]'s lab
  • GP736 (''sinR::tetR'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
  • 1S97 (''sinR''::''phleo''), [Pubmed|8422983], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]
  • GP1672 (''[protein|search|sinR]-[protein|search|tasA]''::''cat'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
  • GP1663 (''[protein|search|yqhG]-[protein|search|sinI]-[protein|search|sinR]-[protein|search|tasA]''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]: pGP1083 , available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP1930 (aphA3) based on [protein|search|pAC7], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP960 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 20395361,20541494,23353768,24988880

    • Modelling of the [protein|search|SinI]/[protein|search|SinR] switch

  • 21095906

    • Original publications

  • 22893383,23378512,23430750,23475644,21856853,21815947,22329926,21326214,21708175,8955328,15661000,8878039,24317403,16923912,15104138,16430695,16430696,18047568,18430133,1906467,7635837,11751836,19201793,10547280,15104138,9799632,19788541,19898538,3125149,8932324,20351052,20923420,8422983,9685500,9158733,23646920,23660663,24256735,24347549,25433524,26283769,26819068