sinR [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

transcriptional regulator (Xre family) of post-exponential-phase responses genes

Locus: BSU24610

Isoelectric point: 7.00

Molecular weight: 12.00 kDa

Function: control of biofilm formation

Product: transcriptional regulator (Xre family)

Essential: no

Synonyms: sin, flaD

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → Transition state regulators
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming

Categories containing this gene/protein

• transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

• AbrB regulon, ScoC regulon, Spo0A regulon

Gene

Coordinates on the chromosome (coding sequence): 2,552,653 -> 2,552,988

Phenotypes of a mutant

• the mutation suppresses the galactose toxicity to a galE mutant PubMed

The protein

Catalyzed reaction/ biological activity

• transcription regulator of biofilm genes, acts as a true repressor of the tapA-sipW-tasA operon and as an anti-activator (prevents binding of the activator protein RemA) of the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon PubMed
• acts as co-repressor for SlrR PubMed

Protein family

• Xre family

Paralogous protein(s)

• SlrR

Domains

• DNA-binding N-terminal domain (aa 1-69) PubMed
• SinI-binding C-terminal domain (aa 74-111) PubMed

Effectors of protein activity

• SinI and SlrA are antagonists to SinR PubMed
• SlrR is also antagonist of SinR PubMed

Structure

• 2YAL (C-terminal domain, aa 74-111) PubMed
• 3QQ6 (N-terminal domain, aa 1-69) PubMed
• 1B0N (complex SinR-SinI) PubMed

Interactions

• SlrA-SinR PubMed, K(D) 10.6 nM PubMed
• SlrR-SinR PubMed, K(D) 47.5 nM PubMed, Veg may inhibit this interaction PubMed
• SinR-SinI PubMed, K(D) 1.8 nM PubMed
• SinR-ScoC

Expression and Regulation

Operon

• sinI-sinR PubMed
• sinR PubMed

Sigma factor

• sinI: SigA PubMed
• sinR: SigA PubMed

Regulation

• sinI: repressed under conditions that trigger sporulation (Spo0A) PubMed
• sinI: repressed during exponential growth (ScoC) PubMed
• sinI: repressed during logrithmic growth (AbrB) PubMed
• sinI: repressed during vegetative growth (SinR) PubMed
• sinR is bimodally expressed and this depends on [rny|search|RNase Y] PubMed

Regulatory mechanism

• Spo0A: transcription repression PubMed
• ScoC: transcription repression PubMed
• AbrB: transcription repression PubMed
• SinR: transcription repression PubMed

Additional information

• the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] (the half-life of the mRNA increases from 3.5 to 13 min) PubMed

Biological materials

Mutant

• GP923 (sinR::spec) PubMed, available in Jörg Stülke's lab
• GP736 (sinR::tetR) PubMed, available in Jörg Stülke's lab
• 1S97 (sinR::phleo), PubMed, available at BGSC
• GP1672 (sinR-tasA::cat) PubMed, available in Jörg Stülke's lab
• GP1663 (yqhG-sinI-sinR-tasA), available in Jörg Stülke's lab

Expression vector

• N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380: pGP1083 , available in Jörg Stülke's lab

LacZ fusion

• pGP1930 (aphA3) based on pAC7, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP960 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Modelling of the SinI/SinR switch

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Original publications

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