hemZ

coproporphyrinogen III oxidase

locus: BSU_09840

pI: 5.90

mw: 57.00 kDa

protein length: 501 aa

gene length: 1506 bp

function: heme biosynthesis

product: coproporphyrinogen III oxidase

essential: no

synonyms: yhaW,yhaV

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]

Gene

Coordinates: 1,057,680 1,059,185

The protein

Protein family

• anaerobic coproporphyrinogen-III oxidase family (with [protein|757C51296E198849EAC1FFA4D37121D9085930A8|HemN], according to UniProt)

Paralogous protein(s)

• [protein|757C51296E198849EAC1FFA4D37121D9085930A8|HemN]

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

• genes: [gene|5A1E474D2A6043ACF0DF00FEA7F66BFB1332C691|hemZ]
  description: [Pubmed|10498703]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10498703], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]: repression, in [regulon|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex regulon]

  regulation

  • induced under anaerobic conditions during growth in the presence of nitrate (no induction in the absence of terminal electron acceptor) ([protein|search|Fnr], [protein|search|ResD], [protein|search|ArfM]) [Pubmed|10498703]

Biological materials

Mutant

• MGNA-B497 (yhaV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1496 NBRP B. subtilis, Japan]
• BKE09840 ([gene|5A1E474D2A6043ACF0DF00FEA7F66BFB1332C691|hemZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATCACCTAATTTA, downstream forward: _UP4_CACTGATTACAGTGCTGCTT
• BKK09840 ([gene|5A1E474D2A6043ACF0DF00FEA7F66BFB1332C691|hemZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATCACCTAATTTA, downstream forward: _UP4_CACTGATTACAGTGCTGCTT

References

• 10498703