prfA

peptide chain release factor 1

Locus: BSU_37010

Isoelectric point: 4.82

Molecular weight: 40.07 kDa

Protein length: 356 aa

Gene length: 1071 bp

Function: translation

Product: peptide chain release factor 1

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation factors
• Groups of genes → Essential genes

Gene

Coordinates: 3,797,085 3,798,155

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG

Protein family

• prokaryotic/mitochondrial release factor family (with PrfB, according to UniProt)

Structure

• 1ZBT (from Streptococcus mutans, 58% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: prfA-prmC-ywkF
  Description: PubMed

• Genes: prfA
  Description: PubMed

References

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