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rpoA [2017-07-05 13:45:58]
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rpoA [2017-07-05 13:45:58]

RNA polymerase alpha subunit
Locus
BSU01430
Isoelectric point
4.59
Molecular weight
34.64 kDa
Protein length
314 aa Sequence Blast
Gene length
942 bp Sequence Blast
Function
transcription
Product
RNA polymerase alpha subunit
Essential
yes
E.C.
2.7.7.6
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
148,931 → 149,875

Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)
  • Protein family

  • RNA polymerase alpha chain family (according to Swiss-Prot)
  • Structure

  • 1Z3E (C-terminal domain, complex with Spx)
  • 3IYD (RNA polymerase from E. coli, 46% identity) PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon
  • Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
  • Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
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    Biological materials

    Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • References

    Reviews

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    Original publications

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