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rpoA [Sun Nov 27 2016 17:53:17 GMT+0100 (CET)]
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rpoA [Sun Nov 27 2016 17:53:17 GMT+0100 (CET)]

RNA polymerase alpha subunit
locus
BSU01430
pI
4.00
mw
34.00 kDa
function
transcription
product
RNA polymerase alpha subunit
essential
yes
ec
2.7.7.6
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|transcription], [SW|essential genes], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|stringent response]

    • Gene

    Coordinates on the chromosome (coding sequence)
    148,931 -> 149,875

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)

    • Protein family

  • [SW|RNA polymerase] alpha chain family (according to Swiss-Prot)

    • Structure

  • [PDB|1Z3E] (C-terminal domain, complex with Spx)
  • [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 46% identity) [Pubmed|19903881]

    • [SW|Interactions]

  • [protein|search|RpoA]-[protein|search|RpoB]-[protein|search|RpoC] [Pubmed|10499798]
  • [protein|search|CcpN]-[protein|search|RpoA] [Pubmed|19726675]
  • [protein|search|RpoA] (C-terminal domain) -[protein|search|Spx] [Pubmed|12642660,22307755]
  • [protein|search|MgsR]-[protein|search|RpoA] [Pubmed|18643936]
  • [protein|search|CcpA]-[protein|search|RpoA] [Pubmed|22512862]
  • [protein|search|RpoA]-[protein|search|CodY] [Pubmed|22512862]
  • [protein|search|SigL]-([protein|search|RpoB]-[protein|search|RpoC]-[protein|search|RpoA]) [Pubmed|26293966]
  • [SW|ComA]-[SW|RpoA] [Pubmed|26582911]

    • Expression and Regulation

    Regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

    • References

    Reviews

  • 22210308

    • Original publications

  • 20084284,19726675,19580872,3093467,2496109,8635744,10675340,12486038,18643936,12642660,16249335,16740936,15378759,26154296,22512862,20817769,22307755,26293966,26582911,19903881