rpoA [Sun Nov 27 2016 17:53:17 GMT+0100 (CET)]

RNA polymerase alpha subunit

Locus: BSU01430

Isoelectric point: 4.00

Molecular weight: 34.00 kDa

Function: transcription

Product: RNA polymerase alpha subunit

Essential: yes

E.C.: 2.7.7.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → RNA polymerase
• Groups of genes → Essential genes

Categories containing this gene/protein

• transcription, essential genes, most abundant proteins

This gene is a member of the following regulons

• stringent response

Gene

Coordinates on the chromosome (coding sequence): 148,931 -> 149,875

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)

Protein family

• RNA polymerase alpha chain family (according to Swiss-Prot)

Structure

• 1Z3E (C-terminal domain, complex with Spx)
• 3IYD (RNA polymerase from E. coli, 46% identity) PubMed

Interactions

• RpoA-RpoB-RpoC PubMed
• CcpN-RpoA PubMed
• RpoA (C-terminal domain) -Spx PubMed
• MgsR-RpoA PubMed
• CcpA-RpoA PubMed
• RpoA-CodY PubMed
• SigL-(RpoB-RpoC-RpoA) PubMed
• ComA-RpoA PubMed

Expression and Regulation

Regulation

• RelA dependent downregulation (Class I) during stringent response PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

References

Reviews

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Original publications

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