sufB

FeS scaffold, synthesis of Fe-S-clusters

locus: BSU_32670

pI: 4.96

mw: 52.56 kDa

protein length: 465 aa

gene length: 1398 bp

function: synthesis of iron-sulfur clusters

product: FeS scaffold

essential: yes

synonyms: yurU

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.3|Biosynthesis of iron-sulfur clusters]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,355,593 3,356,990

Phenotypes of a mutant

• essential [Pubmed|12682299]
• the mutant is viable and Fe-S clusters are dispensable if [protein|search|IspG ]and [protein|search|IspH ]from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate [pubmed|29292548]

The protein

Catalyzed reaction/ biological activity

• synthesis of Fe-S-clusters (the [protein|5991F051C5BAD61A0860BBC64A37DB9D16130998|SufB]-[protein|E966F3808222820C0644DC98F95765B89E6A2551|SufC]-[protein|1A9DA839AB77AF53958CB8426607BEF14F62B85E|SufD] complex) [pubmed|29292548]

Protein family

• UPF0051 (ycf24) family (with [protein|1A9DA839AB77AF53958CB8426607BEF14F62B85E|SufD], according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|5AWF] (from ''E. coli'', the [protein|5991F051C5BAD61A0860BBC64A37DB9D16130998|SufB]-[protein|E966F3808222820C0644DC98F95765B89E6A2551|SufC]-[protein|1A9DA839AB77AF53958CB8426607BEF14F62B85E|SufD] complex, 41% identity) [Pubmed|26472926]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|E966F3808222820C0644DC98F95765B89E6A2551|sufC]-[gene|1A9DA839AB77AF53958CB8426607BEF14F62B85E|sufD]-[gene|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]-[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]-[gene|5991F051C5BAD61A0860BBC64A37DB9D16130998|sufB]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B583 (yurU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1582 NBRP B. subtilis, Japan]

References

Reviews

• 28882201

Original publications

• 17981983,20097860,22383849,15378759,26472926,29292548