ydaO [Fri Dec 09 2016 16:48:52 GMT+0100 (CET)]

putative transporter

locus: BSU04320

pI: 9.00

mw: 66.00 kDa

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|membrane proteins], [SW|proteins of unknown function]

This gene is a member of the following [SW|regulons]

• [SW|YdaO riboswitch]

Gene

Coordinates on the chromosome (coding sequence): 486,432 -> 488,255

The protein

[SW|Localization]

• cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])

Expression and Regulation

Operon

• ''[protein|search|ydaO]'' [Pubmed|22383849]

Regulatory mechanism

• expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding od c-di-AMP [Pubmed|24141192,20511502]

Biological materials

Mutant

• 1A688 (''[protein|search|ydaO]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
• GP93 (''[protein|search|ydaO]''::''cat''), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• BP144 (based on [SW|pAC5]), available in [SW|Fabian Commichau]' and [SW|Jörg Stülke]'s labs

FLAG-tag construct

• GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 25869574

Original publications

• 15096624,20511502,23086297,24141192,25086509,25086507