ydaO [Fri Dec 09 2016 16:40:06 GMT+0100 (CET)]

putative transporter

Locus: BSU04320

Isoelectric point: 9.00

Molecular weight: 66.00 kDa

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• membrane proteins, proteins of unknown function

This gene is a member of the following regulons

• YdaO riboswitch

Gene

Coordinates on the chromosome (coding sequence): 486,432 -> 488,255

The protein

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operon

• ydaO PubMed

Regulatory mechanism

• expression is controlled via termination antitermination by the ydaO riboswitch, expression is switched off upon binding od c-di-AMP PubMed

Biological materials

Mutant

• 1A688 (ydaO::erm), available at BGSC
• GP93 (ydaO::cat), available in Jörg Stülke's lab

Expression vector

• pGP2913 (N-terminal His-tag, expression in E. coli, in pWH844), available in Jörg Stülke's lab

LacZ fusion

• BP144 (based on pAC7), available in Fabian Commichau' and Jörg Stülke's labs

FLAG-tag construct

• GP2405 ydaO-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original publications

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