kimA [2017-05-09 15:36:35]

high-affinity potassium transporter (uptake)

locus: BSU04320

pI: 9.93

mw: 66.60 kDa

protein length: 607 aa

gene length: 1821 bp

function: potassium uptake

product: high-affinity potassium transporter

essential: no

synonyms: ydaO

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 486,432 → 488,255

Phenotypes of a mutant

• a [gene|search|kimA ][gene|search|ktrA ][gene|search|ktrB ]mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751]

The protein

Catalyzed reaction/ biological activity

• uptake of potassium [pubmed|28420751]

[SW|Domains]

• 11 trans-membrane helices at the N-terminus [pubmed|28420751]

[SW|Localization]

• cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])

Expression and Regulation

Operons

• genes: [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP (formed at high potassium concentration) [pubmed|28420751], in [regulon|ydaO riboswitch|ydaO riboswitch]

  regulation

  • expressed at low potassium concentration ([regulon|ydaO riboswitch|ydaO riboswitch]) [Pubmed|28420751]

Biological materials

Mutant

• MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]
• 1A688 (''[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
• GP93 (''[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

Expression vector

• pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• BP144 (based on [SW|pAC5]), available in [SW|Fabian Commichau]' and [SW|Jörg Stülke]'s labs [pubmed|28420751]

FLAG-tag construct

• GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

References

Reviews

• 25869574

Original publications

• 15096624,20511502,23086297,24141192,25086509,25086507,28420751