sucD [Wed Aug 24 2016 13:24:49 GMT+0200 (CEST)]

succinyl-CoA synthetase (alpha subunit)

locus: BSU16100

pI: 5.00

mw: 31.00 kDa

function: TCA cycle

product: succinyl-CoA synthetase (alpha subunit)

essential: no

ec: 6.2.1.5

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

[SW|Categories] containing this gene/protein

• [SW|ATP synthesis], [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,681,617 -> 1,682,519

The protein

Catalyzed reaction/ biological activity

• ATP succinate CoA = ADP phosphate succinyl-CoA (according to Swiss-Prot)

Protein family

• succinate/malate CoA ligase alpha subunit family (according to Swiss-Prot)

Kinetic information

• Reversible Michaelis-Menten [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]

Modification

• phosphorylation on (Ser-19 OR Thr-20) [Pubmed|17218307]

Effectors of protein activity

• Inhibited by 2-oxoglutarate, ATP and NADH [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
• GTP is not accepted by the enzyme [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]

Structure

• [PDB|1JKJ] (E. coli)

[SW|Interactions]

• [protein|search|SucC]-[protein|search|SucD] [Pubmed|20933603]

Additional information

• extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at [http://www.proteopedia.org/wiki/index.php/Succinyl-Coa_Synthetase Proteopedia]

Expression and Regulation

Operon

• ''[protein|search|sucC]''-''[protein|search|sucD]'' [Pubmed|11976317]

Regulation

• repressed by glucose (2.4-fold) ([protein|search|CcpA]) [Pubmed|12850135]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression [Pubmed|12850135]

Additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Biological materials

Mutant

• GP720 (spc), available in [SW|Jörg Stülke]'s lab
• 1A1008 ( ''sucD''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1008&Search=1A1008 BGSC]
• GP791 (''[protein|search|sucC]-[protein|search|sucD]''::''tet''), available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1435 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1425 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

Antibody

• **

References

• 12850135,17218307,11976317,20933603,15378759