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sucD
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sucD

succinyl-CoA synthetase (alpha subunit)
Locus
BSU_16100
Isoelectric point
5.59
Molecular weight
31.23 kDa
Protein length
300 aa Sequence Blast
Gene length
903 bp Sequence Blast
Function
TCA cycle
Product
succinyl-CoA synthetase (alpha subunit)
Essential
no
E.C.
6.2.1.5
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,681,617 1,682,519

The protein

Catalyzed reaction/ biological activity

  • ATP + CoA + succinate --> ADP + phosphate + succinyl-CoA (according to UniProt)
  • Protein family

  • succinate/malate CoA ligase alpha subunit family (single member, according to UniProt)
  • Kinetic information

  • Reversible Michaelis-Menten FEBS Letters
  • Modification

  • phosphorylation on (Ser-19 OR Thr-20) PubMed
  • Effectors of protein activity

  • Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
  • GTP is not accepted by the enzyme FEBS Letters
  • Structure

  • 1JKJ (E. coli)
  • Additional information

  • extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia
  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • repressed by glucose (2.4-fold) (CcpA) PubMed
  • expression is heterogeneous PubMed
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    Biological materials

    Mutant

  • GP720 (spc), available in Jörg Stülke's lab
  • 1A1008 ( sucD::spec), [Pubmed| ], available at BGSC
  • GP791 ∆(sucC-sucD)::tet, available in Jörg Stülke's lab
  • GP2344 ∆(sucC-sucD)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • GP2345 ∆(sucC-sucD::lox72), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • BKE16100 ∆(sucD)::erm trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATTATTTGGTCCCCTGCCT, downstream forward: _UP4_TAATAAAAAAGGGACAGCCG
  • BKK16100 ∆(sucD)::kan trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATTATTTGGTCCCCTGCCT, downstream forward: _UP4_TAATAAAAAAGGGACAGCCG
  • GFP fusion

  • GP1435 (spc, based on pGP1870), available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1425 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

  • **
  • References

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