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gcvH [2017-12-11 13:35:34]
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gcvH [2017-12-11 13:35:34]

glycine cleavage system protein H, 2-oxo acid dehydrogenase, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis
Locus
BSU32800
Isoelectric point
3.89
Molecular weight
14.11 kDa
Protein length
127 aa Sequence Blast
Gene length
381 bp Sequence Blast
Function
glycine utilization
Product
glycine cleavage system protein H
Essential
no
Synonyms
yusH

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,366,123 → 3,366,506

The protein

Protein family

  • gcvH family (according to Swiss-Prot)
  • Cofactors

  • lipoic acid (on Lys-63), lipoylated by LipM PubMed, can probably be removed by SrtN PubMed
  • Structure

  • 3IFT (from Mycobacterium tuberculosis, 50% identity) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-B592 (yusH::erm), available at the NBRP B. subtilis, Japan
  • BKE32800 (ΔgcvH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
  • BKK32800 (ΔgcvH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
  • GP2585 (ΔgcvH::tet comIQ12L) (in DK1042) available at Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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