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gcvH [2017-12-11 13:35:34]
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gcvH [2017-12-11 13:35:34]

glycine cleavage system protein H, 2-oxo acid dehydrogenase, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis
locus
BSU32800
pI
3.89
mw
14.11 kDa
protein length
127 aa Sequence Blast
gene length
381 bp Sequence Blast
function
glycine utilization
product
glycine cleavage system protein H
essential
no
synonyms
yusH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

    • Gene

    Coordinates
    3,366,123 → 3,366,506

    The protein

    Protein family

  • gcvH family (according to Swiss-Prot)

    • [SW|Cofactors]

  • lipoic acid (on Lys-63), lipoylated by [protein|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|LipM] [Pubmed|20882995], can probably be removed by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|SrtN] [pubmed|28900027]

    • Structure

  • [PDB|3IFT] (from ''Mycobacterium tuberculosis'', 50% identity) [Pubmed|20364333]

    • Expression and Regulation

    Operons

    genes
    [gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]-[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]
  • BKE32800 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
  • BKK32800 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
  • GP2585 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::tet comIQ12L) (in DK1042) available at [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 27074917

    • Original publications

  • 20882995,21338421,20364333,28900027