skfE [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

ABC transporter (binding protein), export of the spore killing factor

Locus: BSU01950

Isoelectric point: 6.00

Molecular weight: 26.00 kDa

Function: export of the spore killing factor SkfA

Product: ABC transporter (binding protein)

Essential: no

E.C.: 3.6.3.25

Synonyms: ybdA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
• Lifestyles → Coping with stress → Toxins, antitoxins and immunity against toxins
• Prophages and mobile genetic elements → Prophages → Prophage 1
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• ABC transporters, toxins, antitoxins and immunity against toxins, membrane proteins, prophage 1, sporulation/ other

This gene is a member of the following regulons

• AbrB regulon, PhoP regulon, Spo0A regulon

Gene

Coordinates on the chromosome (coding sequence): 216,913 -> 217,632

Phenotypes of a mutant

• inactivation of skfE reduces sporulation efficiency to 54% that of wild type cells PubMed

The protein

Protein family

• ABC transporter domain (according to Swiss-Prot)

Localization

• cell membrane (according to Swiss-Prot)

Interactions

• SkfE-SkfF PubMed

Expression and Regulation

Operon

• skfA-skfB-skfC-skfE-skfF-skfG-skfH PubMed

Sigma factor

• SigA PubMed

Regulation

• expressed under conditions that trigger sporulation (Spo0A) PubMed
• repressed during logrithmic growth (AbrB) PubMed
• induced by phosphate starvation (PhoP) PubMed

Regulatory mechanism

• Spo0A: transcription activation PubMed
• AbrB: transcription repression PubMed
• PhoP: transcription activation PubMed

Additional information

• Northern blotting during during phosphate limitation showed an intense 0.25 kb 'skfA'-specific transcript, and a weaker 6.5 kb 'skfA-skfB-skfC-skfE-skfF-skfG-skfH' transcript. PubMed

References

Reviews

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Original Publications

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