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skfE
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skfE

ABC transporter (binding protein), export of the spore killing factor
Locus
BSU_01950
Isoelectric point
6.34
Molecular weight
26.75 kDa
Protein length
239 aa Sequence Blast
Gene length
720 bp Sequence Blast
Function
export of the spore killing factor SkfA
Product
ABC transporter (binding protein)
Essential
no
E.C.
3.6.3.25
Synonyms
ybdA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
216,913 217,632

Phenotypes of a mutant

  • inactivation of skfE reduces sporulation efficiency to 54% that of wild type cells PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + sulfate --> ADP + H+ + phosphate + sulfate (according to UniProt)
  • ATP + H2O + thiosulfate --> ADP + H+ + phosphate + thiosulfate (according to UniProt)
  • Protein family

  • ABC transporter superfamily (according to UniProt)
  • Domains

  • ABC transporter domain (aa 4-232) (according to UniProt)
  • Structure

  • 4RVC (from Geobacillus kaustophilus, 33% identity) PubMed
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • PhoP: activation, PubMed, in PhoP regulon
  • Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • Additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb 'SkfA'-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript
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    Biological materials

    Mutant

  • MGNA-C515 (ybdA::erm), available at the NBRP B. subtilis, Japan
  • BKE01950 (skfE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA, downstream forward: _UP4_TAATATCATTTCTTTTTTAT
  • BKK01950 (skfE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA, downstream forward: _UP4_TAATATCATTTCTTTTTTAT
  • References

    Reviews

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    Original Publications

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