skfE

ABC transporter (binding protein), export of the spore killing factor

Locus: BSU_01950

Isoelectric point: 6.34

Molecular weight: 26.75 kDa

Protein length: 239 aa

Gene length: 720 bp

Function: export of the spore killing factor SkfA

Product: ABC transporter (binding protein)

Essential: no

E.C.: 3.6.3.25

Synonyms: ybdA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
• Lifestyles → Coping with stress → Toxins, antitoxins and immunity against toxins
• Prophages and mobile genetic elements → Prophages → Prophage 1
• Groups of genes → Membrane proteins

Gene

Coordinates: 216,913 217,632

Phenotypes of a mutant

• inactivation of skfE reduces sporulation efficiency to 54% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + sulfate --> ADP + H⁺ + phosphate + sulfate (according to UniProt)
• ATP + H₂O + thiosulfate --> ADP + H⁺ + phosphate + thiosulfate (according to UniProt)

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 4-232) (according to UniProt)

Structure

• 4RVC (from Geobacillus kaustophilus, 33% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: skfA-skfB-skfC-skfE-skfF-skfG-skfH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • PhoP: activation, PubMed, in PhoP regulon

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb 'SkfA'-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript

Biological materials

Mutant

• MGNA-C515 (ybdA::erm), available at the NBRP B. subtilis, Japan
• BKE01950 (skfE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA, downstream forward: _UP4_TAATATCATTTCTTTTTTAT
• BKK01950 (skfE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA, downstream forward: _UP4_TAATATCATTTCTTTTTTAT

References

Reviews

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Original Publications

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