bioB

biotin synthase

Locus: BSU_30200

Isoelectric point: 5.55

Molecular weight: 36.77 kDa

Protein length: 335 aa

Gene length: 1008 bp

Function: biosynthesis of biotin

Product: biotin synthase

Essential: no

E.C.: 2.8.1.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of biotin

The protein

Catalyzed reaction/ biological activity

• [sulfur carrier]-SH + dethiobiotin + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)

Protein family

• Radical SAM superfamily (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 1R30 (from E. coli, 36% identity) PubMed

Expression and Regulation

Operons

• Genes: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
  Description: PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in BirA regulon

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

Biological materials

Mutant

• BKE30200 (bioB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGTTCCATCCATTGATTCA, downstream forward: _UP4_TGAAAGAATCAATAAAAGCA
• BKK30200 (bioB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGTTCCATCCATTGATTCA, downstream forward: _UP4_TGAAAGAATCAATAAAAGCA

References

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