yesT

rhamnogalacturonan acetylesterase

locus: BSU_07020

pI: 5.51

mw: 25.80 kDa

protein length: 232 aa

gene length: 699 bp

function: pectin utilization

product: rhamnogalacturonan esterase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]

Gene

Coordinates: 768,137 768,835

The protein

Protein family

• [SW|'GDSL' lipolytic enzyme family] (according to UniProt)

Paralogous protein(s)

• [protein|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|YxiM], [protein|578B79EB64B804FCAACA73AB22458481371A815C|YesY]

Structure

• [PDB|2O14] ([protein|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|YxiM], 29% identity)

Expression and Regulation

Operons

• genes: [gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
  description: [Pubmed|19651770]

  regulatory mechanism

  • [protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR]: activation, [Pubmed|19651770], in [regulon|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR regulon]

  regulation

  • induced by pectin ([protein|search|RhgR]) [Pubmed|19651770]

Biological materials

Mutant

• MGNA-B451 (yesT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1450 NBRP B. subtilis, Japan]
• BKE07020 ([gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATCACATGTTCTC, downstream forward: _UP4_CTTGTGAGCCGGGAGGGAAA
• BKK07020 ([gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATCACATGTTCTC, downstream forward: _UP4_CTTGTGAGCCGGGAGGGAAA

References

• 19651770,17957779,17449691