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lutB [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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lutB [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

lactate catabolic enzyme
locus
BSU34040
pI
5.85
mw
53.00 kDa
function
lactate utilization
product
lactate oxidase
essential
no
synonyms
yvfW
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|FbpB regulon], [SW|FsrA regulon], [SW|LutR regulon], [SW|SinR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,493,519 -> 3,494,958

    Phenotypes of a mutant

  • no growth with lactate as the single carbon source [Pubmed|19201793]

    • The protein

    Catalyzed reaction/ biological activity

  • oxidation of lactate to pyruvate [Pubmed|19201793]

    • Modification

  • phosphorylated on Arg-33 [Pubmed|22517742]

    • [SW|Cofactors]

  • contains an iron-sulfur cluster

    • Expression and Regulation

    Operon

  • ''[protein|search|lutA]-[protein|search|lutB]-[protein|search|lutC]'' [Pubmed|19201793]

    • Regulation

  • repressed by [protein|search|SinR] [Pubmed|19201793]
  • induction by lactate [Pubmed|19201793]
  • less expressed under conditions of extreme iron limitation ([protein|search|FsrA]) [Pubmed|18697947]
  • part of the iron sparing response, down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA], [protein|search|FpbB]) [Pubmed|22427629]

    • Regulatory mechanism

  • [protein|search|SinR]: transcription repression [Pubmed|19201793]
  • dual repression by [protein|search|LutR] (inducer lactate) and [protein|search|SinR] [Pubmed|19201793]
  • [protein|search|FsrA]: translational repression [Pubmed|22427629,18697947]
  • [protein|search|FbpB]: acts as RNA chaperone, increases interaction between ''[protein|search|fsrA]'' and ''[protein|search|lutA]-[protein|search|lutB]-[protein|search|lutC]'' RNAs [Pubmed|22427629]

    • Labs working on this gene/protein

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

    • References

  • 22427629,18697947,16430695,22389480,19201793,19201793,22517742