lutB [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

lactate catabolic enzyme

Locus: BSU34040

Isoelectric point: 5.85

Molecular weight: 53.00 kDa

Function: lactate utilization

Product: lactate oxidase

Essential: no

Synonyms: yvfW

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Categories containing this gene/protein

• utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

• FbpB regulon, FsrA regulon, LutR regulon, SinR regulon

Gene

Coordinates on the chromosome (coding sequence): 3,493,519 -> 3,494,958

Phenotypes of a mutant

• no growth with lactate as the single carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• oxidation of lactate to pyruvate PubMed

Modification

• phosphorylated on Arg-33 PubMed

Cofactors

• contains an iron-sulfur cluster

Expression and Regulation

Operon

• lutA-lutB-lutC PubMed

Regulation

• repressed by SinR PubMed
• induction by lactate PubMed
• less expressed under conditions of extreme iron limitation (FsrA) PubMed
• part of the iron sparing response, down-regulation in a fur mutant (Fur, FsrA, FpbB) PubMed

Regulatory mechanism

• SinR: transcription repression PubMed
• dual repression by LutR (inducer lactate) and SinR PubMed
• FsrA: translational repression PubMed
• FbpB: acts as RNA chaperone, increases interaction between fsrA and lutA-lutB-lutC RNAs PubMed

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

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