thiU

thiamine [SW|ECF transporter] (binding protein)

locus: BSU_13240

pI: 6.05

mw: 21.88 kDa

protein length: 200 aa

gene length: 603 bp

function: thiamine uptake

product: thiamine [SW|ECF transporter] (binding protein)

essential: no

synonyms: ykoF

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.3|Class I ECF transporter]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,391,040 1,391,642

The protein

Structure

• [PDB|1SBR] (complex with thiamine), [PDB|1S99]

[SW|Localization]

• membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|ThiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|ThiX]) [Pubmed|16291685]

Expression and Regulation

Operons

• genes: [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
  description: [Pubmed|16291685]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

  regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]

Biological materials

Mutant

• MGNA-A757 (ykoF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/757 NBRP B. subtilis, Japan]
• BKK13240 ([gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTCCGACCTCCTGTT, downstream forward: _UP4_AGAAAAAACAGAAAGCAGGG

References

• 16291685,12376536,15451668